Tritium Gas Exchange Using a Hydrogen Transfer Catalyst
Tritium gas exchange using a hydrogen transfer catalyst can be employed in labeling compounds such as carbohydrates, nucleosides, nucleotides, alkaloids, amino acids and compounds containing benzylic protons. This technique achieves specific activities of 1-50 Ci/mmol.
Tritium gas exchange using a hydrogen transfer catalyst includes the following:
Exchange with Tritiated Water
Tritiated water in acidic, basic, or neutral solution can be used to label compounds such as nucleotides, pyrimidines and alkaloids. This technique usually achieves specific activities of 0.5-30 Ci/mmol.
Exchange with tritiated water includes the following:
Exposure to Tritium Gas
Labeling by exposure to tritium gas, know as the Wilzbach method, can be performed on some pharmaceutical and other complex compounds with satisfactory results, however many compounds will suffer significant radiation induced decomposition and achieve low specific activities. This method should be used only if the compound cannot be labeled using other less destructive methods.
Gas exposure tritium labeling includes the following:
MB's Ligand Labeling Service is available to researchers who are able to provide the necessary precursor, standards, and purification systems to assist in the custom synthesis of the radiolabeled ligand. The increased level of cooperation between you and MB's radiolabeling staff will in most cases, result in significantly reduced costs.
Catalytic Reduction with Tritium Gas
Catalytic reduction with tritium gas can be used to label compounds that can be obtained in unsaturated form such as aliphatic compounds, steroids, and peptides. This technique usually achieves high specific activities in the range of 30-60 Ci/mmol per double bond reduced and a relatively pure product.
Catalytic reduction with tritium gas includes:
Catalytic Dehalogenation with Tritium Gas
Catalytic dehalogenation with tritium gas can be used to label compounds such as aromatic compounds, alkaloids, nucleosides, nucleotides, peptides, purines and pyrimidines. This technique usually achieves specific activities of 10-30 Ci/mmol per halogen replaced.
Catalytic dehalogenation using tritium gas includes the following:
Reduction with Borohydride, [3H]-
Reduction with sodium or potassium borohydride, [3H]- is employed mainly in the labeling of carbohydrates. This technique usually achieves specific activities between 1-15 Ci/mmol per carbonyl group reduced.
Reduction with borohydride, [3H]- includes the following:
Last Updated on
Tuesday, May 29, 2012 03:14:41 PM