Tritium Labeling Services

• Exchange Reactions with Tritium
• Ligand Labeling Service with Tritium
• Chemical Synthesis with Tritium

Technical Evaluation and Quotation Form

Exchange Reactions

Tritium Gas Exchange Using a Hydrogen Transfer Catalyst

Tritium gas exchange using a hydrogen transfer catalyst can be employed in labeling compounds such as carbohydrates, nucleosides, nucleotides, alkaloids, amino acids and compounds containing benzylic protons. This technique achieves specific activities of 1-50 Ci/mmol.

Tritium gas exchange using a hydrogen transfer catalyst includes the following:

• Exposure of your compound to the appropriate amount of carrier-free tritium gas and catalyst
• Complete removal of labile tritium
• Assay of the crude product for total activity

Exchange with Tritiated Water

Tritiated water in acidic, basic, or neutral solution can be used to label compounds such as nucleotides, pyrimidines and alkaloids. This technique usually achieves specific activities of 0.5-30 Ci/mmol.

Exchange with tritiated water includes the following:

• Reaction of your compound with the appropriate amount of tritiated solvent
• Complete removal of labile tritium
• Assay of crude product for total activity

Exposure to Tritium Gas

Labeling by exposure to tritium gas, know as the Wilzbach method, can be performed on some pharmaceutical and other complex compounds with satisfactory results, however many compounds will suffer significant radiation induced decomposition and achieve low specific activities. This method should be used only if the compound cannot be labeled using other less destructive methods.

Gas exposure tritium labeling includes the following:

• Exposure of your compound to 15 Ci of carrier-free tritium gas
• Complete removal of labile tritium
• Assay of the crude product for total activity

Ligand Labeling Service

 MB's Ligand Labeling Service is available to researchers who are able to provide the necessary precursor, standards, and purification systems to assist in the custom synthesis of the radiolabeled ligand. The increased level of cooperation between you and MB's radiolabeling staff will in most cases, result in significantly reduced costs.

Chemical Synthesis

Catalytic Reduction with Tritium Gas

Catalytic reduction with tritium gas can be used to label compounds that can be obtained in unsaturated form such as aliphatic compounds, steroids, and peptides. This technique usually achieves high specific activities in the range of 30-60 Ci/mmol per double bond reduced and a relatively pure product.

Catalytic reduction with tritium gas includes:

• Reaction of your compound with the appropriate amount of carrier-free tritium gas and catalyst
• Complete removal of labile tritium
• Assay of the crude product for total activity

Catalytic Dehalogenation with Tritium Gas

Catalytic dehalogenation with tritium gas can be used to label compounds such as aromatic compounds, alkaloids, nucleosides, nucleotides, peptides, purines and pyrimidines. This technique usually achieves specific activities of 10-30 Ci/mmol per halogen replaced.

Catalytic dehalogenation using tritium gas includes the following:

• Reaction of your halo compound with the appropriate amount of carrier-free tritium gas and catalyst
• Complete removal of labile tritium
• Assay of crude product for total activity

Reduction with Borohydride, [³H]-

Reduction with sodium or potassium borohydride, [³H]- is employed mainly in the labeling of carbohydrates. This technique usually achieves specific activities between 1-15 Ci/mmol per carbonyl group reduced.

Reduction with borohydride, [³H]- includes the following:

• Reaction of your compound with 100 mCi of borohydride, [³H]-
• Complete removal of labile tritium
• Assay of crude product for total activity

Technical Evaluation and Quotation Form

Last Updated on Tuesday, May 29, 2012 03:14:41 PM
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