Exchange Reactions with Tritium [³H]

Moravek is licensed by the California Department of Public Health, Food and Drug Branch, for the preparation of ³H labeling of active pharmaceutical ingredients (API) for clinical trials*. In addition, Moravek is allowed to handle schedule I-V classified compounds under DEA authority.

Exchange Reactions

Tritium Gas Exchange Using a Hydrogen Transfer Catalyst

Tritium gas exchange using a hydrogen transfer catalyst can be employed in labeling compounds such as carbohydrates, nucleosides, nucleotides, alkaloids, amino acids and compounds containing benzylic protons. This technique achieves specific activities of 1-50 Ci/mmol.

Tritium gas exchange using a hydrogen transfer catalyst includes the following:

Exchange with Tritiated Water

Tritiated water in acidic, basic, or neutral solution can be used to label compounds such as nucleotides, pyrimidines and alkaloids. This technique usually achieves specific activities of 0.5-30 Ci/mmol.

Exchange with tritiated water includes the following:

Exposure to Tritium Gas

Labeling by exposure to tritium gas, known as the Wilzbach method, can be performed on some pharmaceutical and other complex compounds with satisfactory results, however many compounds will suffer significant radiation induced decomposition and achieve low specific activities. This method should be used only if the compound cannot be labeled using other less destructive methods.

Gas exposure tritium labeling includes the following: