Reduction
Catalytic Reduction with Tritium Gas
Catalytic reduction with tritium gas can be used to label compounds that can be obtained in unsaturated form such as aliphatic compounds, steroids, and peptides. This technique usually achieves high specific activities in the range of 30-60 Ci/mmol per double bond reduced and a relatively pure product.
Catalytic reduction with tritium gas includes:
- Reaction of your compound with the appropriate amount of carrier-free tritium gas and catalyst
- Complete removal of labile tritium
- Assay of the crude product for total activity
Reduction with Borohydride, [3H]-
Reduction with sodium or potassium borohydride, [3H]- is employed mainly in the labeling of carbohydrates. This technique usually achieves specific activities between 1-15 Ci/mmol per carbonyl group reduced.
Reduction with borohydride, [3H]- includes the following:
- Reaction of your compound with 100 mCi of borohydride, [3H]-
- Complete removal of labile tritium
- ssay of crude product for total activity
Catalytic Dehalogenation with Tritium Gas
Catalytic dehalogenation with tritium gas can be used to label compounds such as aromatic compounds, alkaloids, nucleosides, nucleotides, peptides, purines and pyrimidines. This technique usually achieves specific activities of 10-30 Ci/mmol per halogen replaced.
Catalytic dehalogenation using tritium gas includes the following:
- Reaction of your halo compound with the appropriate amount of carrier-free tritium gas and catalyst
- Complete removal of labile tritium
- Assay of crude product for total activity